Conservation denotes the percentage of residues that match that of the rCRS among selected species. A query variant is depicted in isolation (i.e., other variants in a test sequence are not shown) and is flanked by rCRS reference for clarity.
| Species | -5 | -4 | -3 | -2 | -1 | 0 | +1 | +2 | +3 | +4 | +5 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Variant at rCRS pos 3460 (locus pos 52) of ND1 | Q | P | F | A | D | T | M | K | L | F | T |
| Homo sapiens (rCRS) | Q | P | F | A | D | A | M | K | L | F | T |
| Alligator mississippiensis | Q | P | F | A | D | G | L | K | L | I | T |
| Artibeus jamaicensis | Q | P | I | A | D | A | V | K | L | F | I |
| Balaenoptera musculus | Q | P | F | A | D | A | I | K | L | F | T |
| Balaenoptera physalus | Q | P | F | A | D | A | I | K | L | F | T |
| Bos taurus | Q | P | I | A | D | A | I | K | L | F | I |
| Canis lupus familiaris | Q | P | I | A | D | A | V | K | L | F | T |
| Cebus albifrons | Q | P | I | A | D | A | M | K | L | F | T |
| Ceratotherium simum | Q | P | I | A | D | A | I | K | L | F | T |
| Cyprinus carpio | Q | P | I | A | D | G | V | K | L | F | I |
| Dasypus novemcinctus | Q | P | I | A | D | A | I | K | L | F | I |
| Didelphis virginiana | Q | P | F | A | D | A | L | K | L | F | I |
| Drosophila melanogaster | Q | P | F | C | D | A | I | K | L | F | T |
| Equus asinus | Q | P | I | A | D | A | L | K | L | F | I |
| Equus caballus | Q | P | I | A | D | A | L | K | L | F | I |
| Erinaceus europaeus | Q | P | I | A | D | A | V | K | L | F | T |
| Felis catus | Q | P | I | A | D | A | V | K | L | F | T |
| Gallus gallus | Q | P | V | A | D | G | V | K | L | F | I |
| Glis glis | Q | P | I | A | D | A | M | K | L | F | I |
| Gorilla gorilla | Q | P | F | A | D | A | M | K | L | F | T |
| Halichoerus grypus | Q | P | I | A | D | A | V | K | L | F | T |
| Hippopotamus amphibius | Q | P | F | A | D | A | I | K | L | F | T |
| Hylobates lar | Q | P | F | A | D | A | M | K | L | F | T |
| Loxodonta africana | Q | P | I | A | D | A | I | K | L | F | T |
| Macaca sylvanus | Q | P | F | A | D | A | M | K | L | F | T |
| Macropus robustus | Q | P | I | A | D | A | V | K | L | F | T |
| Mus musculus | Q | P | F | A | D | A | M | K | L | F | M |
| Nycticebus coucang | Q | P | F | A | D | A | M | K | L | F | I |
| Ornithorhynchus anatinus | Q | P | I | A | D | A | V | K | L | F | T |
| Oryctolagus cuniculus | Q | P | I | A | D | A | I | K | L | F | T |
| Ovis aries | Q | P | I | A | D | A | I | K | L | F | I |
| Pan paniscus | Q | P | F | A | D | A | M | K | L | F | T |
| Pan troglodytes | Q | P | F | A | D | A | M | K | L | F | T |
| Papio hamadryas | Q | P | F | A | D | A | M | K | L | F | T |
| Phoca vitulina | Q | P | I | A | D | A | V | K | L | F | T |
| Pongo abelii | Q | P | F | A | D | A | L | K | L | F | T |
| Pongo pygmaeus | Q | P | F | A | D | A | L | K | L | F | T |
| Rattus norvegicus | Q | P | F | A | D | A | M | K | L | F | M |
| Rhinoceros unicornis | Q | P | I | A | D | A | I | K | L | F | T |
| Sciurus vulgaris | Q | P | F | A | D | A | M | K | L | F | I |
| Sus scrofa | Q | P | I | A | D | A | L | K | L | V | T |
| Talpa europaea | Q | P | I | A | D | A | V | K | L | F | T |
| Tarsius bancanus | Q | P | F | A | D | A | M | K | L | F | I |
| Vicugna pacos | Q | P | I | A | D | A | I | K | L | F | T |
| Xenopus laevis | Q | P | I | A | D | G | V | K | L | F | I |