Conservation denotes the percentage of residues that match that of the rCRS among selected species. A query variant is depicted in isolation (i.e., other variants in a test sequence are not shown) and is flanked by rCRS reference for clarity.

For the 13 protein-coding loci, amino acid alignments are shown in their transcribed orientation. All but ND6 are read from the 'top' or 'light' (L-) strand; ND6 is read from the reverse complement (the 'bottom' or 'heavy' (H-) strand of the DNA).
For the 22 tRNA and 2 rRNA loci, nucleotide alignments are shown. The majority are read from the L-strand; 8 tRNAs (Q, A, N, C, Y, S1, E, and P) are read in reverse orientation from the complementary H-strand. However, by convention all variants are reported using the light strand nucleotides for comparison with the rCRS and are shown here.

Species	-5	-4	-3	-2	-1	0	+1	+2	+3	+4	+5
Variant at rCRS pos 14495 (locus pos 60) of ND6 (light strand)	V	F	L	I	Y	S	G	G	M	M	V
Homo sapiens (rCRS)	V	F	L	I	Y	L	G	G	M	M	V
Alligator mississippiensis	V	L	L	V	Y	L	G	G	L	L	V
Artibeus jamaicensis	V	F	L	I	Y	L	G	G	M	L	V
Balaenoptera musculus	V	F	L	I	Y	L	G	G	M	L	V
Balaenoptera physalus	V	F	L	I	Y	L	G	G	M	L	V
Bos taurus	V	F	L	I	Y	L	G	G	M	M	V
Canis lupus familiaris	V	F	L	I	Y	L	G	G	M	L	V
Cebus albifrons	V	F	L	I	Y	L	G	G	M	M	V
Ceratotherium simum	V	F	L	I	Y	L	G	G	M	L	V
Cyprinus carpio	L	F	L	I	Y	L	G	G	M	L	V
Dasypus novemcinctus	V	F	L	V	Y	L	G	G	M	L	V
Didelphis virginiana	V	F	L	V	Y	L	G	G	M	L	V
Drosophila melanogaster	L	F	L	I	F	L	G	G	M	L	V
Equus asinus	V	F	L	I	Y	L	G	G	M	L	V
Equus caballus	V	F	L	I	Y	L	G	G	M	L	V
Erinaceus europaeus	V	F	L	I	Y	L	G	G	M	L	V
Felis catus	V	F	L	I	Y	L	G	G	M	L	V
Gallus gallus	L	F	L	V	Y	L	G	G	M	L	V
Glis glis	V	F	L	I	Y	L	G	G	M	L	V
Gorilla gorilla	V	F	L	I	Y	L	G	G	M	M	V
Halichoerus grypus	V	F	L	I	Y	L	G	G	M	L	V
Hippopotamus amphibius	V	F	L	V	Y	L	G	G	M	L	V
Hylobates lar	V	F	L	I	Y	L	G	G	M	M	V
Loxodonta africana	V	F	L	V	Y	L	G	G	M	M	V
Macaca sylvanus	M	F	L	V	Y	L	G	G	M	M	V
Macropus robustus	V	F	L	I	Y	L	G	G	M	L	V
Mus musculus	V	F	L	I	Y	L	G	G	M	L	V
Nycticebus coucang	V	F	L	V	Y	L	G	G	M	L	V
Ornithorhynchus anatinus	V	F	L	V	Y	L	G	G	M	L	V
Oryctolagus cuniculus	M	F	L	I	Y	L	G	G	M	L	V
Ovis aries	V	F	L	I	Y	L	G	G	M	M	V
Pan paniscus	V	F	L	I	Y	L	G	G	M	M	V
Pan troglodytes	V	F	L	I	Y	L	G	G	M	M	V
Papio hamadryas	M	F	L	I	Y	L	G	G	M	M	V
Phoca vitulina	V	F	L	I	Y	L	G	G	M	L	V
Pongo abelii	V	F	L	I	Y	L	G	G	M	M	V
Pongo pygmaeus	V	F	L	V	Y	L	G	G	M	M	V
Rattus norvegicus	V	F	L	I	Y	L	G	G	M	L	V
Rhinoceros unicornis	V	F	L	I	Y	L	G	G	M	L	V
Sciurus vulgaris	V	F	L	I	Y	L	G	G	M	L	V
Sus scrofa	V	F	L	I	Y	L	G	G	M	L	V
Talpa europaea	V	F	L	I	Y	L	G	G	M	L	V
Tarsius bancanus	V	F	L	I	Y	L	G	G	M	L	V
Vicugna pacos	V	F	L	I	Y	L	G	G	M	M	V
Xenopus laevis	L	F	L	I	Y	L	G	G	M	L	V

Variant at rCRS pos 14495 (locus pos 60) of ND6 (light strand)

Homo sapiens (rCRS)

Alligator mississippiensis

Artibeus jamaicensis

Balaenoptera musculus

Balaenoptera physalus

Bos taurus

Canis lupus familiaris

Cebus albifrons

Ceratotherium simum

Cyprinus carpio

Dasypus novemcinctus

Didelphis virginiana

Drosophila melanogaster

Equus asinus

Equus caballus

Erinaceus europaeus

Felis catus

Gallus gallus

Glis glis

Gorilla gorilla

Halichoerus grypus

Hippopotamus amphibius

Hylobates lar

Loxodonta africana

Macaca sylvanus

Macropus robustus

Mus musculus

Nycticebus coucang

Ornithorhynchus anatinus

Oryctolagus cuniculus

Ovis aries

Pan paniscus

Pan troglodytes

Papio hamadryas

Phoca vitulina

Pongo abelii

Pongo pygmaeus

Rattus norvegicus

Rhinoceros unicornis

Sciurus vulgaris

Sus scrofa

Talpa europaea

Tarsius bancanus

Vicugna pacos

Xenopus laevis

Conservation grid